Molecular Characterization,Expression Profiles,and Association Analysis of Bovine MYOT Gene With Meat Production Traits
In a previous screen myotilin (MYOT) gene was described to be one of the genes involved in determining adult”s muscle phenotypes and showed increasing expression in bovine fetus” muscle over developmental time.Based on the fact that differentially expressed genes in early stages of muscle development are potential candidate genes for meat quality improvement and production efficiency, we have decided to study how bovine MYOT gene could regulate meat production.Here, this gene was sequenced; its expression profile and association with meat quality and quantity traits were also analyzed.We successfully cloned the full-length of bovine MYOT gene cDNA long of 2234 bp using skeletal muscle as tissue sample.Real-time PCR revealed that MYOT mRNA was expressed in few tissues with highest expression levels in skeletal muscle followed by heart and heart fat.Bioinformatics analysis showed that bovine MYOT protein contains two potential Ig-like domain profile, three N-glycosylation sites, eight protein kinase C phosphorylation sites, six potential Casein kinase Ⅱ phosphorylation sites, one tyrosine kinase phosphorylation site, seven N-myristoylation sites, one Endoplasmic reticulum targeting sequence and one cell attachment sequence site.Association analysis revealed that T152C SNP was associated with loin muscle area, back fat thickness and Intra-muscular fat (P < 0.05) in Chinese Qinchuan cattle breed with c allele being the beneficial one.Those findings showed that Myot gene is essential for muscle regulation and his SNPs can be used as genetic marker for molecular-assisted selection in cattle breeding.
Bovine MYOT gene Expression pattern Bioinformatics study, single nucleotide polymorphisms (SNP) association analysis
Linsen Zan C.Adoligbe Yifan Huang-fu
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R.China;National Beef Cattle Improvement Center of Northwest A&F University, Yangling, Shaanxi, P.R.China
国内会议
第二届中国肉牛选育改良与产业发展国际研讨会暨中国畜牧兽医学会养牛分会八届二次学术研讨会
陕西杨凌
英文
84-92
2013-10-22（万方平台首次上网日期，不代表论文的发表时间）