Association Analysis Of Bovine Foxa2 Gene Single Sequence Variant and Haplotype Combinations With Growth Traits in Chinese Cattle
Forkhead box A2 (Foxa2) has been recognized as one of the most potent transcriptional activator that is implicated in the control of feeding behavior and energy homeostasis.However, similar researches about the effects of genetic variations of Foxa2 gene on growth traits are lacking.Therefore, this study detected Foxa2 gene polymorphisms by DNA pool sequencing, PCR-RFLP and PCR-ACRS methods in 822 individuals from three Chinese cattle breeds.The results showed that four sequence variants (SVs) were screened, 3including two mutations (SV1, g.7005 C>T and SV2, g.7044 C>G) in intron 4, one mutation (SV3, g.8449 A>G) in exon 5 and one mutation (SV4, g.8537 T>C) in the 3”UTR.Notably, association analysis of the single mutations with growth traits in total individuals (at 24 months) revealed that significant statistical difference were found in four SVs, and SV4 locus was highly significantly associated with growth traits throughout all three breeds (P<0.05 or P<0.01).Meanwhile, haplotype combination CCCCAGTC also indicated remarkably associated to better chest girth and body weight in Jiaxian Red cattle (P<0.05).We herein described a comprehensive study on the variability of bovine Foxa2 gene that was predictive of molecular markers in cattle breeding for the first time.
Association Cattle Foxa2 gene Growth traits Haplotype combination Sequence variant
Mei Liu Hong Chen Mijie Li Shaoqiang Wang Yao Xu Xianyong Lan Xiukai Cao Zhuanjian Li Chuzhao Lei Chunlei Zhang
College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Mole Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu, P.R.China 22
国内会议
第二届中国肉牛选育改良与产业发展国际研讨会暨中国畜牧兽医学会养牛分会八届二次学术研讨会
陕西杨凌
英文
166-176
2013-10-22（万方平台首次上网日期，不代表论文的发表时间）