Novel Polymorphisms of the APOA2 Gene and its Promoter Region Affect Body Traits in Cattle
Apolipoprotein A-Ⅱ (APOA2) is one of the major constituents of High-density lipoprotein and plays a critical role in lipid metabolism and obesity.However, similar research for the bovine APOA2 gene is lacking.In this study, polymorphisms of the bovine APOA2 gene and its promoter region were detected in 1021 cows from four breeds by sequencing and PCR-RFLP methods.Totally, we detected six novel mutations which included one mutation in the promoter region, two mutations in the exons and three mutations in the introns.There were four polymorphisms within APOA2 gene were analyzed for the low frequencies of other two mutations.The allele A, T, T and G frequencies of the four loci were predominant in the four breeds when in separate or combinations analysis which suggested cows with those alleles to be more adapted to the steppe environment.The association analysis indicated three SVs in Nangyang cattle, two SVs in Qinchun cattle and the 9 haplotypes in Nangyang cattle were significantly associated with body traits (P<0.05 or P<0.01).The results of this study suggested the bovine APOA2 gene is possible a strong candidate gene for body traits in the cattle breeding program.
Apoa2 gene Association Bovine Body traits Haplotype
Yang Zhou Caixia Li Hanfang Cai Yao Xu Xianyong Lan Chuzhao Lei Hong Chen
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100 China
国内会议
第二届中国肉牛选育改良与产业发展国际研讨会暨中国畜牧兽医学会养牛分会八届二次学术研讨会
陕西杨凌
英文
204-210
2013-10-22（万方平台首次上网日期，不代表论文的发表时间）