Expression Pattern,Polymorphism and Association Analysis of CDIPT Gene on Qinchuan Cattle
CDIPT (CDP-diacylglycerol-inositol 3-phos-phatidyltransferase, EC 2.7.8.11), an integral membrane protein, was found on the cytoplasmic side of the endoplasmic reticulum and the Golgi apparatus.It performed the last step in the de novo biosynthesis of PtdIns.In recent years, PtdIns has been considered as an essential role in energy metabolism, fatty acid metabolic pathway and intracellular signal transduction in eukaryotic cells.In this studay, Real-Time PCR analysis showed that the expression of CDIPT gene was remarkably different in diverse tissues.We also detected the polymorphism of bovine CDIPT gene and analyzed its association with body measurement and meat quality traits of Qinchuan cattle.Blood samples were taken from a total of 638 Qinchuan cattle aged from 18-24 months.DNA sequencing and PCR-RFLP were used to find out CDIPT gene single nucleotide polymorphism (SNP).Three SNPs g.244T>C (NCBI: rs42069760), g.1496G>A and g.1514G>A were found in this study.g.244T>C located at 5”untranslated region (5”UTR) of exon 1 and showed three genotypes: TT, TC and CC.g.1496G>A and g.1514G>A detected the first time were located in intron 3 and showed the same genotypes: GG, GA and AA.Analysis results showed that the polymorphism of these 3 SNPs was significantly associated with body measurement traits (BMTs) and meat quality traits (MQTs).Based on the results of this study, it is inferred that CDIPT gene may have potential effects on BMTs and MQTs and could be used for marker-assisted selection.
CDIPT Qinchuan cattle PCR-RFLP SNP
Changzhen Fu Hong Wang Yaokun Li Jiali Wang Gong Cheng Hongbao Wang Wucai Yang Linsen Zan
College of Animal Science and Technology of Northwest A&F University, P.R.China, 712100 College of Animal Science and Technology of Northwest A&F University, P.R.China, 712100;National Bee
国内会议
第二届中国肉牛选育改良与产业发展国际研讨会暨中国畜牧兽医学会养牛分会八届二次学术研讨会
陕西杨凌
英文
218-226
2013-10-22（万方平台首次上网日期，不代表论文的发表时间）