Expression Analysis of Gli1 and Gli2 in Different Tissues and Muscle Derived Cells of Qinchuan Cattle

摘要：

　　The Hedgehog signaling pathway regulates the differentiation of many kinds of ceils and plays a critical role in many embryonic or post-natal development processes.Glil and Gli2 are two transcription factors of Hh signaling pathway.In this study, we used the quantitative real-time PCR to detect the relative expression of Glil and Gli2 in 13 tissues from three two-year-old purebreed Qinchuan cattle, and also in different cell populations derived from muscle and different stages of myogenic differentiation of myoblast.The expressions of Glil and Gli2 in muscle are the lowest in the 13 tissues (P < 0.05), and they declined predominantly from ppl cell to pp6 cell.However, the expression of Gli2 elevated during the myogenic differentiation until the 6th day.We speculated that Hh signaling was negatively activated in myocytes and quiescent myoblast.The up going expression of Glil and Gli2 at the early days of myogenic differentiation suggested that Hh signaling would be activated when the quiescent bovine myoblast was stimulated to initiate the myogenic differentiation.

关键词： Hedgehog signaling pathway Myoblast Myogenic differentiation Quantitative real-time PCR

作者: Yang Liu Linsen Zan Hongbao Wang Gong Cheng Bijie Jiang

作者单位: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R.of China College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R.of China;

会议类型: 国内会议

会议名称: 第二届中国肉牛选育改良与产业发展国际研讨会暨中国畜牧兽医学会养牛分会八届二次学术研讨会

会议地点: 陕西杨凌

会议语种:英文

页码: 227-233

在线出版日期: 2013-10-22（万方平台首次上网日期，不代表论文的发表时间）