Molecular Characterisation and Fibrolytic Activities of Neocallimastix and Orpinomyces Fungi Obtained From the Rumen Contents of Wild Yaks (Bos Grunniens)
Fourteen fungal isolates were obtained with a Hungate”s roll-tube technique from rumen contents of wild yaks (Bos grunniens) and they were identified into fungal groups of the monocentric genus Neocallimastix and the polycentric genus Orpinomyces based on their morphological characteristics and internal transcribed spacer region 1 sequences.Acetyl esterase (AE, 168.15 vs.140.49 mU), ferulic acid esterase (FAE, 4.94 vs.3.44 mU) and xylanase (658.36 vs.620.28 mU) activities were higher in the Neocallimastix group than in the Orpinomyces group.Enzyme activity of FAE was highly correlated with activities of AE (r=0.79, P<0.0001) and xylanase (r=0.65, P=0.0008) while the lowest coefficient occurred between AE and xylanase (r=0.52, P=0.0051).In brief, anaerobic fungi exhibited higher fibrolytic activities in the genus Neocallimastix than those activities in the genus Orpinomyces in the rumen contents of wild yaks, suggesting these fungi might play an important role in fibre degradation in the rurnen.
Wild yak Rumen fungi Phylogenetic diversity Ferulic acid esterase Acetyl esterase Xylanase
YangChun Cao HongJian Yang
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultur State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultur
国内会议
第二届中国肉牛选育改良与产业发展国际研讨会暨中国畜牧兽医学会养牛分会八届二次学术研讨会
陕西杨凌
英文
306-317
2013-10-22（万方平台首次上网日期，不代表论文的发表时间）