Detecting Chemical Signals in the Giant Panda (Ailuropoda melanoleuca) urine

摘要：

　　Olfactory communication plays an important role in the sociality of giant pandas (Ailuropoda melanoleuca).A wealth of literature concerning the behavioral and chemical aspects of giant panda scent marking is available (Schaller et al., 1985;Swaisgood et al.,1999; Swaisgood et al., 2000; Swaisgood et al., 2002; White et al.,2002; Hagey and MacDonald, 2003; White et al., 2003; Tian, 2004; Liu et al., 2006;Tian, 2007; Liu et al., 2008; Zhang et al., 2008), but results vary.The number and type of chemical components in scent markings varies between studies due to different pre-treatments and analysis methods (Liu et al., 2000; Hagey and MacDonald, 2003; Yuan et al., 2004; Goodwin et al., 2005; Zhang et al., 2008).To address this issue we used a mixed headspace-solid phase micro-extraction (HS-SPME) and gas chromatography-mass spectrometry to pre-treat and detect the chemical composition of panda urine from crude urine samples from four captive giant pandas.We found 82 peaks and through searching the mass spectrometry library of the National Institute of Standards and Technology, 46 different compounds were putatively identified.These components might act as pheromones in the chemical communication between different individuals.Our results indicate that a HS-SPME combined GC-MS method is effective for detecting and identifying chemical compounds in crude giant panda urine and could be used for other species.

关键词： Giant Panda Urine Headspace-Solid Phase Microextraction (HS-SPME) Gas Chromatography-Mass Spectrometry (GC/MS) Chemical Signal

作者: Yuliang Liu Qiuhong Xin Dingzhen Liu Zhigang Cai Zhihe Zhang Jingchao Lan Rongping Wei Desheng Li Rong Hou

作者单位: Key Laboratory for reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province Ministry of Education,Key Laboratory of Biodiversity Science and Ecological Engineering,College of L Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province

会议类型: 国内会议

会议名称: 2010大熊猫繁育技术委员会年会

会议地点: 福州

会议语种:英文

页码: 110-112

在线出版日期: 2010-11-11（万方平台首次上网日期，不代表论文的发表时间）