Anti-tumor Immune Response of Folate-Conjugated Chitosan Nanoparticles Containing the IP-10 Gene in Mice with Hepatocellular Carcinoma
(0) Folate-chitosan nanoparticles (FA-CS) were loaded with mouse interferon-inducible protein-10 (IP-10) plasmid DNA to generate FA-CS-IP-10,which was used for immunotherapy in hepatocellular carcinoma (HCC).H22 tumor-bearing mice were treated with FA-CS-IP-10 and tumor targeting was observed by in vivo imaging.Flow cytometry was used to detect the number of myeloid-derived suppressor cells (MDSCs) in tumor and CD4+CD25+FoxP3+ T-regulatory cells (CD4+CD25+FoxP3+ Tregs) in spleen.The enzyme-linked immunospot assay was used to quantify the FA-CS-IP-10-induced tumor-specific cytotoxic T lymphocytes (CTLs) and secretory IFN-γ.IP-10 expression,tumor vessel density,cell proliferation and apoptosis were evaluated by immunohistochemistry.FA-CS-IP-10 significantly inhibited tumor growth,suppressed angiogenesis,increased apoptosis of cancer cells,and prolonged the survival time of H22 tumor-bearing mice.Tumor tissue from FA-CS-IP-10-treated mice had significantly higher IP-10 expression,a lower proportion of Tregs in spleen and higher proportion of MDSCs in tumor and up-regulation of IFN-γ secretion in CTLs compared to the other experimental groups.FA-CS-IP-10 is a promising immunotherapeutic agent for HCC.
immunotherapy FA-CS-IP-10 hepatocellular carcinoma IP-10
Chunhui LAI Yongxiang ZHAO Xiaoling LU Xia YU Yuan XIE Jian HE Yi PENG Sufang ZHOU Xiaoxun XIE Guorong LUO Nuo ZHOU
National Center for International Research of Biological Targeting Diagnosis and Therapy,Guangxi Med National Center for International Research of Biological Targeting Diagnosis and Therapy,Guangxi Med National Center for International Research of Biological Targeting Diagnosis and Therapy,Guangxi Med
国内会议
昆明
英文
1-15
2014-05-24（万方平台首次上网日期，不代表论文的发表时间）