超高液相色谱-蒸发光检测器法分析蜂蜜中的寡聚麦芽糖含量
We report an ultra performance liquid chromatography (UPLC) separation with evaporative lightscattering detection (ELSD) method for the rapid and direct analysis of six maltooligosacc6arides. UPLCseparation employing a mobile phase with pure water and acetonitrile containing 0.2％ triethylamine resulted inenhanced separation and a running time of less than 6 minutes. Only a simple liquid-liquid extraction was requiredfor the sample pretreatment. This UPLC-ELSD method provided limits of quantifications (LOQs) from 15.0--25.0μg/mL for the six maltooligosaccharides ranging from 15 to 2000 pg/mL and showed excellent linearity(R2≥0.9914). This optimized method provided a satisfactory infra-day precision of 2.4 ％-9.4 ％,inter-dayprecision of 5.5 ％-12.1％，accuracy of -7.7％-+11.4％at each level. We have successfully applied this method todetermine the six maltooligosaccharide concentrations in honey samples from apiaries and supermarkets.Concentrations of six maltooligosaccharides in 290 honey samples varied from undetectable to 14.1 mg/mL andthe lowest concentration that could be detected by the developed method was 0.132 mg/mL. This studydemonstrates the potential application of the UPLC-ELSD technique for sensitive monitotoring of the authenticity ofhoney samples in the food processing industry.
蜂蜜 寡聚麦芽糖 含量测定 超高液相色谱 蒸发光检测器
周金慧 赵静 李熠 吴黎明 薛晓锋 张金振 陈芳 金玥 王鹏 黄京平
中国农业科学院蜜蜂研究所;农业部蜂产品质量监督检验测试中心(北京);农业部蜂产品质量安全风险评估实验室
国内会议
第十届海峡两岸蜜蜂与蜂产品学术研讨会暨首届全国蜂产业高峰论坛
扬州
中文
221-230
2013-11-03（万方平台首次上网日期，不代表论文的发表时间）