Optimization of Microwave-Assisted Saponification (MAS) for Simultaneous Determination of 1-Octacosanol and 1-Triacontanol in Beeswax by Fast Gas Chromatography
Determination of contents of 1-octacosanol and 1-triacontanol in natural wax of plant and animal origin are essentially important for the study of their beneficial physiological activities and health benefits.In this paper, a robust, fast and effective analytical method for simultaneous determination of 1-octacosanol and 1-triacontanol in beeswax was established and validated based on fast gas chromatography combined with flame ionization detector (FID).A sample pretreatment coupled with microwave-assisted saponification and non-derivatization prior to analysis has been optimized.Compared with conventional GC and traditional saponification, the proposed method was less time-consuming, more effective and more environmentally friendly due to involving an HP-1MS fast capillary column and microwave irradiation and non-derivatization step.The method had a good linearity over the range 1-500 mg/L with good linear regression correlation coefficients above 0.997, and showed an excellent intra-day and inter-day precision (RSD≤3.8%).The recovery was 86.5%-110.2% (RSD≤5.04%).The limit of quantitation (LOQ) (S/N =10) of this method was determined to be 5 mg/L and 8 mg/L for 1-octacosanol and 1-triacontanol, respectively.
Microwave-Assisted Saponification (MAS) Fast Gas Chromatography 1-Octacosanol 1-Triacontanol
Qiang Wang Xiaofeng Xue Yi Li Liming Wu Jing Zhao
Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China ;R
国内会议
第十届海峡两岸蜜蜂与蜂产品学术研讨会暨首届全国蜂产业高峰论坛
扬州
英文
292-298
2013-11-03（万方平台首次上网日期，不代表论文的发表时间）