Simultaneous determination of aflatoxins and ochratoxin A in bee pollen by low-temperature fat precipitation and immunoaffinity column cleanup coupled with LC-MS/MS
A simple and sensitive method has been developed for simultaneous determination of aflatoxins (AFB 1, AFB2, AFG1 and AFG2) and ochratoxin A (OTA) in bee pollen.The analytes in the sample were extracted with a mixture of acetonitrile/water (60:40, v/v), using low-temperature for fat precipitation, followed by immunoaffinity column clean up of extracts.The mycotoxins were quantified using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with an electrospray ionisation (ESI) and a triple quadrupole (QqQ) analyser.Matrix effect, accuracy and precision were evaluated and achieved good results calculated by matrix-matched calibration standards which reduced the influence of matrix effect.Recoveries at three levels were in the range of 74.3-96.5% with RSD less than 10.0%.The correlation coefficients (r2) of the five mycotoxins were higher than 0.997.The method showed high sensitivity with LOD below 0.05 μg/kg.
bee pollen immunoaffinity column low-temperature fat precipitation aflatoxins ochratoxin A
XiaoFeng Xue Liming Wu Yi Li
Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100093, P.R.China Risk Assessment Laboratory for Bee Products Quality and Safety of Ministry of Agriculture, Beijing 1
国内会议
第十届海峡两岸蜜蜂与蜂产品学术研讨会暨首届全国蜂产业高峰论坛
扬州
英文
387-395
2013-11-03（万方平台首次上网日期，不代表论文的发表时间）