Suitable Internal Control MicroRNA Genes for Measuring MiRNA Abundance in Pig Milk During Different Lactation Periods
Determination of an optimal set/number of internal control microRNA (miRNA) genes is a critical, but often undervalued, detail of quantitative gene expression analysis.No validated internal genes for miRNA quantitative PCR (q-PCR) in pig milk were available.We compared the expression stability of six porcine internal control miRNA genes in pig milk from different lactation periods (one hour, three days, seven days, 14 days, 21 days and 28 days postpartum), using an EvaGreen q-PCR approach.We found that using the three most stable internal control genes to calculate the normalization factor is sufficient for producing reliable q-PCR expression data.We also found that miRNAs are superior to ribosomal RNA (rRNA) and snRNA, which are commonly used as internal controls for normalizing miRNA q-PCR data.In terms of economic and experimental feasibility, we recommend the use of the three most stable internal control miRNA genes (miR-17,-107 and-103) for calculating the normalization factors for pig milk samples from different lactation periods.These results can be applied to future studies aimed at measuring miRNA abundance in porcine milk.
Pig miRNA Normalization Milk Quantitative PCR
Yiren Gu Yan Liang Jianjun Gong Kai Zeng Xuemei Yang Xuebin Lv
Sichuan Animal Science Academy, ChengduSichuan 610066
国内会议
郑州
英文
46-49
2013-09-01（万方平台首次上网日期，不代表论文的发表时间）