NTE Treatment Results in Increased Fpn Expression in Hippocampus of Rats Subjected to Cerebral Ischemia
Objective: To observe the expression ofFerroportin (Fpn) at different time points in rats after focal cerebral ischemia treated with or without Chinese medicine NTE (extract of HuangQi, Chuan Xiong, Di Long, Jiang Can)_Methods: The experiment is carried out by two steps: Firstly , SD rats were randomly divided into 2 h, 6 h, 12 h, 24 h, 72 h groups after operation of Middle cerebral artery occlusion (MCAO), mRNA and protein level of Fpn were detected by immunohistochemistry and RT-PCR at above time points; Secondly, the rats were randomly allotted five groups as following: sham operation group, model group, low dose group of NTE (3 g/kg), medial dose group of NTE (9_g/kg), large dose group of NTE (27 g/kg).After three days of corresponding therapy by intragastric administration once a day, the regional cerebral ischemia model was reproduced by middle cerebral artery occlusion (MCAO)with suture method.Three days later, the rats treated by previous method.The third day, Neurological behavior of rats were detected by neurobehavioral testing.Fpn in Hippocampal C2 region was measured by immunohistochemical method and mRNA of Fpn was detected by RT-PCR.Results: Expression of Fpn in hippocamp C2 reaches the highest point at 12 h after operation (P<0.05); compared with model group, the large dose group of NTE (27 g/kg) displays a lower Neurological behavior score (P<0.05); and large dose group ofNTE showes higher lever expression of Fpn both at mRNA and protein level compared with sham operation group and model group (P<0.05).Conclusion: Disregulation of intracellular iron balance perhaps is the new mechanism of cerebral ischemia injury, the NTE can protect the neuron in Hippocampal C2 area by adjusting the expression of Fpn to balance the iron after cerebral ischemia.
Ferroportin (Fpn) Naotaifang extract(NTE) cerebral ischemia
Jun Liao Xing Xia Guo-zuo Wang Yong-mei Shi Jin-wen Ge
Department of Anatomy, Hunan University of Chinese Medicine Changsha 410208, Hunan Province, China Campus Network Center,Hunan University of Chinese Medicine Changsha 410208, Hunan Province, China College of Integrated Medicine, Hunan University of Chinese Medicine, Hunan Province Key Laboratory
国内会议
北京
英文
356-366
2013-11-01（万方平台首次上网日期，不代表论文的发表时间）