高通量测序在发热死亡病人血清中发现新基因型TTV病毒
Torque teno virus (TTV) has been found world-wide prevalent in healthy population and patients with various diseases, but its ethological role had not been determined yet. Using high throughput unbiased sequencing to screen viruses in serum of a patient with persistent high fever who died of suspected viral infection and prolonged weakness, we identified the complete genome sequence of a TTV Virus (isolate rebel-1).The genome of TTV Hebel-1 is 3649bp, which encodes 4 putative open reading frames and has a G+C content of 49％. Genomic comparison and BLASTN search found that the assembled genome of the TTV-Hebel-1 represents a novel group with genome sequence highly heterologous to other reported TTV stains. Phylogenetic tree constructed with complete genome sequence Showed that TTV Hebel-1 arid an uncharacterized Taiwan Strain TW53A37 constitute a new TTV genotype. S1nGe the patient is high suspected of viral 1nfeGtlOn arid filed eventually without other known causes, and no virus other than the novel TTV is identified in his serum sample, we supposed that this virus might be the etiological agents of his disease. Although the direct causal link between the novel TTV genotype infection and the patient”s disease has not been confirmed yet, surveillance of the novel TTV genotype is advised and its role in disease deserves to be explored.
发热死亡患者 转矩特诺病毒 高通量测序
米志强 苑鑫 裴广倩 王伟 安小平 彭帆 李莎莎 柏长青 童贻刚
军事医学科学院微生物流行病研究所,北京100071 解放军第307医院呼吸科,北京100071 军事医学科学院微生物流行病研究所,北京100071;解放军第307医院呼吸科,北京100071
国内会议
重庆
中文
138-149
2013-11-01（万方平台首次上网日期，不代表论文的发表时间）