Analysis of Polychlorinated Biphenyls Congeners in Soil Samples by Real-time Immuno-PCR assay
A novel real-time immuno-PCR (RT-IPCR) assay for the determination of 3,4-dichlorinated biphenyl (IUPAC PCB12) is described in this paper.Using the optimized assay,the linear range for the determination of PCB12 was found to be 10.0 fg mL-1 to 1.0 ng mL-1 with a correlation coefficient of 0.987 and a detection limit of 1.53 fg mL-1.The RT-IPCR assays were tested for their cross-reactivity profiles using four selected congeners and four Aroclor products.The assays were highly specific for the congeners; however,they were less specific for Aroclor 1242.The RT-IPCR results for the soil samples correlated with the concentrations of PCBs obtained by gas chromatography/mass spectrometry.
3,4-dichlorinated biphenyl Polyclonal antibody Real-time immuno-PCR
Chen Han-yu Zhuang Hui-sheng
School of Environmental Science and Engineering,Shanghai Jiao Tong University,Shanghai,200240,China;Institute of Environmental Monitoring and Assessment,Shanghai JiaoTong University,Shanghai 200240,China
国内会议
南宁
英文
23-24
2013-10-01（万方平台首次上网日期，不代表论文的发表时间）