Development of a novel dual-functional lateral flow assay for on-site detection of clenbuterol
Recently,more sensitive methods for analysis of clenbuterol (CL) are required,because of potential risk and illegal use of CL as a hazard to human health.Lateral flow assays (LFAs) have been used as a reliable,rapid,and cost effective on-site screening technique for the simultaneous determination of CL.However,the available lateral flow assay for CL requires a competitive format,leading to low sensitivity of detection when judged by naked eyes.In this study,we reported a novel dual-functional LFA for monitoring CL in terms of fluorescence-quenching effect of gold nanoparticles (AuNPs).The most important feature of this novel LFA is its dual function as a fluorescence assay under excitation light,but under natural light as an AuNPs LFA.It is apparent that signal intensity of the T-line is associated with elevated level of CL.In the “turn on” mode when results observed by naked eyes with excitation light,the sensitivity of detection reached 0.04 ng/mL.Whereas,in “turn off” mode using a traditional AuNPs LFAs,results are determined under natural light,the sensitivity decreased to 5 ng/mL.This novel LFAs could be used for a variety of small analytes such as pesticides,persistent organic pollutants,explosives,toxins,medicinal and abused drugs,hormones,etc.
Lateral flow assays Dual-functional Fluorescence quenching Clenbuterol
FU Qiangqiang ZHOU Kenan LAN Caifeng LIANG Jiajie SHI Congying TANG Yong
Department of Bioengineering,Guangdong Province Key Laboratory of Molecular Immunology and Antibody Department of Bioengineering,Guangdong Province Key Laboratory of Molecular Immunology and Antibody
国内会议
南宁
英文
203-205
2013-10-01（万方平台首次上网日期，不代表论文的发表时间）