Preparative separation of alkaloids from Aconitum carmichaeli by pH-zone-refining counter-current chromatography
pH-zone-refining counter-current chromatography was successfully applied to the separation of mesaconitine (Ⅰ), aconitine (Ⅱ) and hypaconitine (Ⅲ) from Aconitum Carmichaeli Debx.A 2.3 g quantity of sample was separated using the following two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water (5∶5∶1∶9, v/v), 10 mM triethylamine in organic stationary phase and 10 mM hydrochloric acid in aqueous mobile phase.45 mg of mesaconitine (Ⅰ), 202 mg of aeonitine (Ⅱ) and 53 mg of hypaconitine (Ⅲ) were obtained from 2.3 g of crude extract in a separation.The purities of compounds Ⅰ, Ⅱ, and Ⅲ were 91.0%, 95% and 96%, respectively, as determined by HPLC.The chemical structures of these compounds were identified by ESI-MS and 1H-NMR.
pH-zone-refining counter-current chromatography Aconitum carmichaeli alkaloids
Liu Dahui Shu Xikai Liu Wei Wang Xiao Yang Bin
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences,Kunming 650231, China Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan,Shandong 25
国内会议
昆明
英文
365-365
2012-03-14（万方平台首次上网日期，不代表论文的发表时间）