Effects on Rapid Propagation in Different Explants and Medium of Wild Lilium leuanthum
”Objective” To screen out the optimal medium and explant for tissue cuhure of wild Lilium leuanthum.”Method”The tissue culture of wild Lilium leuanthum was studied using its scales, root pieces, stem pieces and leaves as explants respectively.The basic mediums were MS, Bζ and White.Four differential mediums were: MS+2.00 mg/L 6-BA+0.20mg/L NAA, MS+0.20mg/L 6-BA+1.00 mg/L IAA, MS+0.10 mg/L KT+0.10 mg/LNAA and MS+0.03 mg/L NAA.Six proliferation formulas were as below: MS+0.03mg/L NAA, MS+2.00 mg/L 6-BA+0.20mg/L NAA, MS+0.20 mg/L 6-BA+1.00 mg/L IAA,MS+0.10mg/L KT, MS+020 mg/L KT+0.20 mg/L NAA and MS+0.10 mg/L KT+0.15 mg/L NAA.Four rooting media were: MS+1.00 mg/L IBA, MS+1.00 mg/L IBA+0.20 mg/L 6-BA, 1/2MS+1.00 mg/L IBA+0.20 mg/L 6-BA and White+1.00 mg/L IBA+0.20mg/L 6-BA.”Result” MS medium was the optimum basic medium.The optimal differential mediums for scale of wild Lilium leuanthum was MS +0.10 mg/L KT+0.10 mg/L NAA, for root pieces and stem pieces of wild Liliumn leuanthum were MS+ 2.00 mg/L 0-BA +0.20 mg/L NAA, for leaf pieces was MS +0.10 mg/L KT+0.10 mg/L NAA, respectively.The optimum subculture medium was MS+ 0.10 mg/L KT +0.15 mg/L NAA and the optimal medium for rooting of rootless bulbs and seedlings was 1/2MS+0.20 mg/L 6-BA+1.00 mg/L IBA.In the field, the tissue culture seedlings formed by scales and roots grew more strongly than others.”Conclusion” The optimal tissue culture mediums and explants for wild Lilium leuanthum were obtained in this work, which provided basis for the rapid propagation of wild Lilium leuanthum.
Wild Lilium leuanthum Tissue culture Rapid propagation Explants Scale China
PAN You-zhao ZHAO Jin-ping ZENG Xiang-miao LI Chun-feng ZHAO Yu-ying
School of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, China Jinzhou Institute of Forestry Science, Jingzhou, Hubei 434025, China Taihu Farm of Jinzhou, Jingzhou, Hubei 434025, China
国内会议
重庆
英文
300-303
2011-12-14（万方平台首次上网日期，不代表论文的发表时间）