Comparison Between RAPD and AFLP Markers in Analyzing the Genetic Diversity of Cultivars and Its Application in Potato Breeding
The genetic diversity of Kexin series potato cultivars, and the application of RAPD and AFLP markers to the practice of potato breeding were studied using two methods.Seven primer combinations were used in the analysis of AFLP, and 54-90 bands were amplified in each lane, whereas 4-9 bands were amplified by each RAPD primer.In RAPD, the genetic distance of Kexin potato cuitivars was between 0.1707-0.7222 and the average value was 0.3917.In AFLP, the genetic distance was between 0.2091-0.7679 and the average value was 0.4811.The Shannon-weaver index and Simpson index from AFLP were all higher than those from RAPD, indicating that the genetic diversity identified by AFLP was higher than that by RAPD.The cluster analysis based on RAPD and AFLP showed that Kexin series potato cultivars could be divided into three groups.Both markers proved in the molecular level that the genetic basis of the Kexin series potato cuitivars was wider.From the results, it was observed that: RAPD was more suitable to detect the genetic distance and analyze the kinship of potato cultivars because it was a simple, fast, and low-cost technique.However, the AFLP markers were more suitable to reveal the difference of potato cultivars and identify the important breeding resource materials.
potato RAPD AFLP cluster analysis genetic diversity
LI Feng-yun DONG Quan-zhong SHENG Wan-min LIU Zhao-jun TIAN Guo-kui LI Qing-quan WANG Li-chun LOU Shu-bao XU Hong-yan WANG Yan
Keshan Branch of Heilongjiang Academy of Agricultural Sciencs, Keshan 161606 Biology Research Center, Heiiongjiang Academy of Agricultural Sciencs, Harbin 150086
国内会议
深圳
英文
283-294
2011-11-09（万方平台首次上网日期，不代表论文的发表时间）