Identification of Naegleria species in environmental water samples from Northeastern China
Naegleria is a free-living amoeba,and pathogenic Naegleria may pose a health risk to people exposed to recreational waters.The objective of the present study was to determine if there are pathogenic amoebae in environmental water samples collected from Northeastern China.During July to September 2012,a total of 70 water samples were collected from Changchun,Northeastern China,and Naegleria was enriched by in vitro culture and detected by PCR using Naegleria genus-specific primers.Naegleria was detected in 65 (92.9%) of 70 water samples.DNA sequence and phylogenetic analyses based on the internal transcribed spacer (ITS) rDNA sequences revealed four Naegleria species,including N.pagei (n =24) and N.Australiensis (n =18),N.clarki (n =13) and N.gruberi (n =10),in which N.australiensis is pathogenic to mice.This is the first report on Naegleria species in Northeastern China,showing that almost all environmental water samples were contaminated with Naegleria,including N.pagei,N.Australiensis,N.clarki and N.grnberi,which should be considered a potential public health threat.
Northeastern China Naegleria PCR identification
Wei Wang Lili Cao Feng Wei Quan Liu
Military Veterinary Institute,Academy of Military Medical Sciences,666 Liuying Xilu,Changchun 130122 Military Veterinary Institute,Academy of Military Medical Sciences,666 Liuying Xilu,Changchun 130122 College of Life Science,Jilin Agricultural University,2888 Xincheng Street,Changchun,130118,Jilin Pr
国内会议
中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
郑州
英文
24-27
2013-11-01（万方平台首次上网日期，不代表论文的发表时间）