An Eimeria Vaccine Candidate Based on Eimeria tenella Immune Mapped Protein 1 and the TLR-5 agonist Salmonella typhimurium FliC Flagellin
In this study,IMPI in Eimeria tenella was predicted to be a membrane protein,consistent with the transmembrane domain prediction result.To evaluate immunogenicity of IMPI in Eimeria tenella,a chimeric subunit vaccine consisting of E.tenella IMP 1 (EtIMP 1) and a molecular adjuvant (a truncated flagellin,FliC) was constructed and over:expressed in E.coli and its efficacy against E.tenella infection was evaluated.ThreE -week:old AA broiler chickens were vaccinated with the recombinant EtIMP 1:truncated FliC without adjuvant or EtIMP1 with Freund” s Complete Adjuvant.Immunization of chickens with the recombinant EtIMP 1: truncatedFliC fusion protein resulted in stronger cellular immune responses than immunization with only recombinant EtIMP 1 with adjuvant.The clinical effect of the EtIMP 1:truncated FliC without adjuvant was also greater than that of the EtIMP 1 with adjuvant,which was evidenced by the differences between the two groups in body weight gain,oocyst output and caeeal lesions of E.tenella:challenged chickens.The results suggested that the EtIMP 1:?agellin fusion protein can be used as an effective immunogen in the development ofsubunit vaccines against Eimeria infection.This is the first demonstration of antigen:specific protective immunity against avian coccidiosis using a recombinant Flagellin as an apicomplexan parasite vaccine adjuvant in chickens.
Eimeria tenella Immune mapped proteinl Flagellin Vaccine
Guangwen Yin Xun Suo Mei Qin Xianyong Liu Zhijian Huang Qian Han Jingxia Suo Xinming Tang Geru Tao Wenxue Wu
College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China National Animal Protozoa Laboratory & College of Veterinary Medicine,China Agricultural University,B National Animal Protozoa Laboratory & College of Veterinary Medicine,China Agricultural University,B Department of Biochemistry,Virginia Tech,Blacksburg,VA 24061,USA
国内会议
中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
郑州
英文
27-34
2013-11-01（万方平台首次上网日期，不代表论文的发表时间）