Protective immunity against Toxoplasma gondii induced by DNA immunization with gene encoding a novel vaccine candidatE-calcium:dependent protein kinase 3
In the present study,we constructed a DNA vaccine expressing T.gondii CDPK3 (TgCDPK3) and evaluated its protective efficacy against T.gondii in Kunming mice.The gene sequence encoding TgCDPK3 was inserted into the eukaryotic expression vector pVAX I,and immunized mice intramuscularly.The results showed that mice immunized with pVAX:CDPK3 developed a high level of.speci?c antibodies and a strong lymphoproliferative response.The signi?cantly increased levels ofIFN: γ,IL:2,IL: 12 (p70) and IL:23 and high ratio of IgG2a to IgG1 antibody titers indicated that a Th 1 type response was elicited after immunization with pVAX:CDPK3.Furthermore,the percentage ofCD4+ T cells in mice vaccinated with pVAX:CDPK3 was significantly increased.After lethal challenge using the virulent RH strain ofT.gondii tachyzoites,the mice immunized with pVAX:CDPK3 prolonged the survival time from 10 days to24 days (13.5 ± 4.89) compared to untreated mice or those received PBS or pVAX I within 7 days (P < 0.05).In chronic infection model (10 cysts ofT.gondii PRU strain),the numbers of brain cysts of the mice in pVAX:CDPK3 group reduced signi?cantly when compared with those in control groups (P < 0.05),and the rate of reduction could reach to about 50%.TgCDPK3 can generate protective immunity against T.gondii infection in Kunming mice and is a promising vaccine candidate for further development of an effective vaccine against T.gondii.
Toxoplasma gondii Toxoplasmosis TgCDPK3 DNA vaccine Protective immunity
张念章 黄思扬 周东辉 陈佳 徐颖 田维鹏 路静 朱兴全
中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,兰州 730046
国内会议
中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
郑州
英文
64-71
2013-11-01（万方平台首次上网日期，不代表论文的发表时间）