Distribution of lethal giant larvae (Lgl) protein in the tegument and negative impact of siRNA:based gene silencing on worm surface structure and egg hatching in Schistosoma japonicum
Lethal giant larvae (Lgl) is evolutionarily conserved tumor suppressor present in fungi and animals.It plays an essential role in establishing apical-basal cell polarity,cell proliferation,differentiation and tissue organization.Here we report the presence ofLgl gene in the blood fluke Schistosomajaponieum (SjLgl),and the chief distribution of SjLgl protein in the unique surface tegument structure.The expression of SjLgl could be knocked down in the parasite in vitro and in vitro by a short interfering RNA (siRNA):based RNA interference approach with a simple soaking method or by tail vein injection into the infected mice.Additionally,SjLgl:speci -tic siRNA treatment during the infection in mice significantly altered the surface structure of adult worm,and negatively impacted egg hatching rate.These observations implied that Lgl may be explored as a novel target for developing immuno: and/or small moleculE-based therapeutics to control and treat the infections caused by schi -stosome and other flatworms.
Lethal giant larvae Schistosoma japonicum RNA interference egg hatching rate
Yufan Cao Yanli Shi Hongbin Qiao Yunxia Yang Jinming Liu Yaojun Shi Jiaojiao Lin Guan Zhu Yamei Jin
Key Laboratory of Animal Parasitology,Ministry of Agriculture of China,Shanghai Veterinary Research College of Veterinary Medicine,Northeast Agricultural University,Harbin,China Department of Veterinary Pathobiology,College of Veterinary Medicine & Biomedical Sciences,Texas A&M
国内会议
中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
郑州
英文
99-105
2013-11-01(万方平台首次上网日期,不代表论文的发表时间)