Proteomic analysis of tegument:exposed proteins of female and male Schistosoma japonicum worms

摘要：

　　The interplay between sexes is a prerequisite for female growth,reproductive maturation and egg pro -duction,and the basis of schistosome pathopoiesis and propagation.The tegument is in direct contact with the host environment and its surface membranes are particularly crucial for schistosome survival in the definitive host.In this study,a streptavidin:biotin affinity purification technique combined with LC:MS/MS was used to analyze putative tegument:exposed proteins in female and male adult Schistosoma j aponicum worms.In total,179 proteins were identified in females and 300 in males,including 119 proteins common to both sexes,and 60 female biased and 181 male biased proteins.Some (e.g.,serpin and CD36:like class B scavenger receptor) were involved in host:schistosome interactions,while some (e.g.,gynecophoral canal protein) were important in the in terplay between sexes.Gene Ontology analysis revealed that proteins involved in protein glycosylation and lyso -some were highly expressed in females,while proteins involved in intracellular signal transduction,regulation ofactin filament polymerization and proteasome core complex were highly expressed in males.These results might elucidate physiological differences between sexes.Our study provide new insights into schistosome grow -th and sexual maturity in the final host and permit the screening of vaccine candidates or drug targets for schist -osomiasis.

关键词： Schistosoma japonicum tegument:exposed proteins, proteome female and male

作者: Min Zhang Jiaojiao Lin Yang Hong Yanhui Han Hongxiao Han Jinbiao Peng Chunhui Qiu Jianmei Yang Ke Lu Zhiqiang Fu

作者单位: Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of An Shanghai Public Health Clinical Center,Fudan University,Shanghai 201508,China College of Life Sciences,Fujian Agriculture and Forestry University,FuZhou,Fujian Province 350002,P.

会议类型: 国内会议

会议名称: 中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会

会议地点: 郑州

会议语种:英文

页码: 106-115

在线出版日期: 2013-11-01（万方平台首次上网日期，不代表论文的发表时间）