Characterization of Dicrocoelium dendriticum isolates from small ruminants in Shaanxi Province,northwestern China,using internal transcribed spacers of nuclear ribosomal DNA
The genetic variations in internal transcribed spacers (ITS) spanning ITS: 1,5.8S and ITS:2 rDNA of Dicrocoelium dendriticum,isolated from sheep and goats in four geographical regions in Shaanxi province,we -re examined.The lengths of ITS: 1,5.8S and ITS:2 rDNA sequences for D.dendriticum were 749 bp,161 bp,and 234 bp,respectively.Intra:specific sequence variations ofD.dendriticum were 0:0.5% for ITS: 1,and 0:1.3% for ITS:2 rDNA,while the inter:specific variations among species in genus Dicrocoelium in ITS:2 rDNA were 3.4: 12.3%.Phylogenetic analysis based on sequences of ITS:2 rDNA showed that all D.dendriticum isolates in the present study were grouped with reference D.dendriticum isolates from sheep and goat,and D.dendriticum iso -lates from cattle and Japanese serow were clustered in a sister clade.However,the phylogenetic tree could not reveal geographically genetic relationships of D.dendriticum isolates in different origins and hosts.These find -ings provided basic information for further study of molecular epidemiology and control of D.dendriticum inf -ection in Shaanxi province as well as in the world.
Dicrocoelium dendriticum genetic variations internal transcribed spacers phylogenetic analysis
Bian Qingqing Zhao Guanghui Song Junke Fang Yanqin Yu Sanke Lin Qing
College of Veterinary Medicine,Northwest A &F University,Yangling,Shaanxi Province 712100,PR China
国内会议
中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
郑州
英文
115-118
2013-11-01（万方平台首次上网日期，不代表论文的发表时间）