Flavin-containing monooxygenases mediated metaflumizone resistance in beet armyworm, Spodoptera exigua
Flavin-containing monooxygenases (FMO) are the phase Ⅰ metabolism enzymes playing important role like CYPs in the oxidation of most nitrogen-, sulfur-, and phosphorus-containing chemical xenobiotics.The physiology characteristics and functions of FMOs were intensively investigated in mammalian systems; however there were scarce study in insects.Our research showed esterase contributed to metaflumizone resistance and first unveiled the role of FMO in insecticide detoxification and resistance Spodoptera exigua.The FMO inhibitor, methimazole, potentiated the toxicities of metaflumizone on resistant larvae of this species, the synergism ratio of methimazole on resistant populations collected from Huizhou, Guangdong province, China, in 2011 (resistant ratio =941.7) and 2012 (resistant ratio =60.3) were 3.1 and 1.9, respectively.The enzymatic assays also revealed higher FMO activities in resistant populations than in susceptible strain, and successive selection with metaflumizone further increased the FMO activities in the field resistant population.The higher FMO activities in elder larval stage and in midgut of larvae signify the importance of FMO in detoxification to xenobiotic from food sources.The synergism assay and FMO activity analysis suggested that FMO take part in the metaflumizone detoxification in resistant populations of S.exigua and conferred the metaflumizone resistance in S.exigua.A novel mechanism for insecticide resistance by insect was proposed.
Flavin-containing monooxygenases Insecticide resistance Metaflumizone Methimazole Spodoptera exigua
Xiangrui Tian Xingxing Sun Jianya Su
College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China
国内会议
南京
英文
249-259
2013-08-01（万方平台首次上网日期，不代表论文的发表时间）