Genetic Diversity of Bartonella quintana in Macaques Suggests Zoonotic Origin of Trench Fever
Bartonella quintana is a bacterium that causes a broad spectrum of diseases in humans including trench fever.Humans were previously considered to be the primary,if not the only reservoir hosts for B.quintana.In order to identify the animal reservoir and extend our understanding of the ecological and evolutionary history of B.quintana,we examined blood samples from macaques and performed multi-locus sequence typing (MLST) analysis.We demonstrated the prevalence of B.quintana infection was common in macaques from main primate centers in mainland China.Overall,18.0% (59/328) of rhesus macaques and 12.7% (39/308) of cynomolgus macaques were found to be infected with B.quintana by blood culture and/or polymerase chain reaction.The infection was more frequently identified in juvenile and young monkeys compared to adult animals.In contrast with the relatively low level of sequence divergence of B.quintana reported in humans,our investigation revealed much higher genetic diversity in non-human primates.We identified 44 new nucleotide variable sites and 14 novel sequence types (STs) among the B.quintana isolates by MLST analysis.Some STs were found only in cynomolgus macaques,while some others were detected only in rhesus macaques,suggesting evidence of host-cospeciation,which were further confirmed by phylogenetic analysis and splits decomposition analysis.Our findings suggest that trench fever may primarily be a zoonotic disease with macaques as the natural hosts.
Bacteria Bartonella quintana MLST macaque trench fever zoonotic disease
Jie-Ying Bai Xiao-Fei Zhang Lin Zeng
Laboratory Animal Center,Academy of Military Medical Sciences,Beijing,People”s Republic of China
国内会议
中国畜牧兽医学会家畜传染病分会第八次全国会员代表大会暨第15次学术研讨会
徐州
英文
1199-1203
2013-10-21（万方平台首次上网日期，不代表论文的发表时间）