实时研究蛋白质和光动力学疗法中光敏剂的相互作用
This paper described a new assay for real-time investigation on the interaction between protein and photosensitizer of photodynamic therapy (PDT) using a quartz crystal microbalance (QCM) biosensor. The new generation photosensitizer, meso-tetrakis(4-hydroxyphenyl)porphyrin (p-THPP), was immobilized on the gold electrode of the QCM chip by combining sol-gel and self-assembly method. The binding interactions of the immobilized p-THPP with various proteins in solution were monitored as the frequency variations of the modified sensor. Such a rapid screen analysis of interactions showed that p-THPP-immobilized sensor was sensitive and specific interaction only with hemoglobin (Hb)(Fig.1).
恶性肿瘤 光动力学疗法 蛋白质 光敏剂
李峰
北京大学化学与分子工程学院,北京,100871
国内会议
北京
中文
63-63
2009-01-01（万方平台首次上网日期，不代表论文的发表时间）