Chemical profile and anti-carcinogenic effects of different polar saponins fractions from leaves of Panax notoginseng
To evaluate the chemical profile and anti-carcinogenic effect amongst totalsaponins fraction (TSF),25% ethanolic fraction (25EF),50% ethanolic fraction (50EF) and85% ethanolic fraction (85EF) by macroporous resin from leaves of Panax notoginseng.The simultaneous determination of 13 main sapoins,as well as full-scale chemical profileof different fractions,was analyzed by HPLC-DAD and LC-ESI-MSn.The anti-proliferativeeffects were determined on KP4 cells (human pancreatic cancer cells),NCI-H727cells(human lung cancer),HepG2 cells (human hepatocellular cancer) and SGC-7901 (humangastric adenocarcinoma) by cytotoxic assay.Chemical analysis indicated that 85EFpossessed the most abundant anti-tumor protopanaxdiol saponins,including markersaponins:F2,20 (R)-Rg3,20 (S)-Rg3 and Rh2.Besides,the results of MTT assay showedthat 85EF had the strongest anti-tumor effects among the four fractions.25EF showed theweakest anti-proliferative effects,while 50EF and TSF exhibited slight increasing inanti-proliferative activity.From the points of comprehensive utilization of resource,85EF,enriched with low polar PPD group saponins,is a new alternative source of anti-cancersaponins and a promising novel useful botanical product for cancer themoprevention.
Leaves of Panax notoginseng Different polar saponins fraction Chemical profile Anti-carcinogenic effects
Qian Mao Yi Li Jie Yang Pinghu Zhang Qiang Wang
Department of Metabolomics and Key laboratory of New Drug Delivery System of Chinese Materia Medica, State Laboratory of Modern Chinese Medicines,China Pharmaceutical University,Ministry of Education,N Nanjing Normal University Center for Analysis and Testing,Nanjing Normal University,Nanjing210009,P. Jiangsu Center for New Drug Screening & National New Drug Screening Laboratory,China Pharmaceutical
国内会议
南京
英文
1-25
2012-11-02（万方平台首次上网日期，不代表论文的发表时间）