Somatic embryogenesis and organogenesis in Dendrocalamus hamiltonii
In this study,mature zygotic embryos and plant growth regulators to regenerate were used plantlets of the bamboo species Dendrocalamus hamiltonii.Incubating explants in Murashige and Skoog (MS) medium supplemented with 1.0-3.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) induced callus formation.Optimal shoot differentiation and subsequent shoot growth was also obtained in the MS medium supplemented with 2 mg/L benzyladenine (BA),1 mg/L kinetin (KT),and 1 mg/L naphthaleneacetic acid (NAA).Root induction was enhanced by 5 mg/L indole-3-butyric acid (IBA).Histological analysis demonstrated that somatic embryogenesis and organogenesis were induced during callus initiation,shoot differentiation,and development of plantlets from the mature zygotic embryos.Our data provides a useful means for bamboo plant regeneration.
Dendrocalamus hamiltonii Zygotic embryo Embryogenesis Organogenesis
Ning Zhang Wei Fang Yan Shi Qianqian Liu Haiyun Yang Renyi Gui Xinchun Lin
The Research Center for the State Key Laboratory of Subtropical Silviculture, Zhejiang Forestry University,Zhejiang, 311300
国内会议
南昌
英文
317-323
2010-09-01（万方平台首次上网日期，不代表论文的发表时间）