Genetic diversity assessment and ex situ conservation strategy of the endangered Dendrobium officinale (Orchidaceae) using new trinucleotide microsatellite markers
Dendrobium officinale (Orchidaceae) is an endangered plant species with important medicinal value.To evaluate the effectiveness of ex situ collection of D.officinale genetic diversity,we developed 15 polymorphic trinucleotide microsatellite loci of D.officinale to examine the genetic diversity and structure of three D.officinale germplasm collections comprising 120 individuals from its germplasm collection base and their respective wild populations consisting of 62 individuals from three provinces in China.The three germplasm collections showed reductions in gene diversity and average number of alleles per locus,but an increase in average number of rare alleles (frequency ≤ 0.05) per locus in comparison with their wild populations.However,the differences in gene diversity between the germplasm collections and wild populations were not statistically significant.The analysis using STRUCTURE revealed evident differences in genetic composition between each germplasm collection and its wild population,which probably because the D.officinale individuals with distinct genotypes in each wild population were unevenly selected for establishing its germplasm collection.For conservation management plans,we propose the D.officinale individuals with rare alleles need to be conserved with a top priority,and those individuals with the most common alleles also should be concerned.The 15 new microsatellite loci may be used as a powerful tool for further evaluation and conservation of the genetic diversity of D.officinale germplasm resources.
Dendrobium officinale Genetic diversity Trinucleotide microsatellite Ex situ collection
Beiwei Hou Jing Luo Xiaoyu Ding
College of Life Sciences,Nanjing Normal University,No. 1,Wenyuan Road,Nanjing 210046,China
国内会议
南京
英文
1-11
2012-09-01（万方平台首次上网日期，不代表论文的发表时间）