The Anopheles community and the role of Anopheles minimus on malaria transmission on the China-Myanmar border
Background: Malaria around China-Myanmar border is a serious health problem in the countries of South-East Asia.An.minimus is a principle malaria vector with a wide geographic distribution in this area.Malaria is endemic along the boundary between Yurman province in China and the Kachin State of Myanmar where the local Anopheles community (species composition) and the malaria transmission vectors have never been clarified.Methods: Adult Anopheles specimens were collected using CDC light traps in four villages along the border of China and Myanmar from May 2012 to April 2013.Morphological and molecular identification of mosquito adults confirmed the species of Anopheles.Blood-meal identification using the female abdomens was conducted using multiplex PCR.For sporozoite detection in An.minimus,sets of 10 female salivary glands were pooled and identified with SSU rDNA using nested PCR.Monthly abundance of An.minimus populations during the year was documented.The diversity of Anopheles and the role ofAn minimus on malaria transmission in this border area were analyzed.Results: 4,833 adult mosquitoes in the genus Anopheles were collected and morphologically identified to species or species complex.The Anopheles community is comprised of 13 species, and 78.83% of our total specimens belonged toAn.minimus s.l., followed byAn.maculatus (5.55%) and the An.culicifacies complex (4.03%).The quantity of trapped An.minimus in rainy season of malaria transmission was greater than during the non-malarial dry season, and a peak was found in May 2012.An.minimus fed on the blood of four animals: humans (79.8%), cattle (10.6%), pigs (5.8%) and dogs (3.8%).1,500 females of An.minimus were pooled into 150 samples and tested for sporozoites: only 1 pooled sample was found to have sporozoites ofPlasmodium vivax.Conclusion: Anopheles is abundant with An.minimus being the dominant species and having a high human blood index along the China-Myanmar border.The sporozoites in An.minimus were determined to be Plasmodium vivax with 0.07-0.7% infection rote.
Anopheles community An.minimus blood meal Plasmodium vivax China-Myanmar border
Guo Yu Bin Chen Guiyun Yan Naixin Zhang Daibin Zhong Ying Wang Zhengbo He Zhentian Yan Wenbo Fu Feilong Yang
Institute of Entomology and Molecular Biology,College of Life Sciences,Chongqing Normal University,C Program in Public Health,College of Health Sciences,University of California Irvine,California,USA Department of Pathogenic Biology,College of Medicine,Third Military Medical University,Chongqing,Chi
国内会议
重庆
英文
1-14
2013-10-01（万方平台首次上网日期，不代表论文的发表时间）