Localization and expression of Hsp27 and α B-crystallin in rat primary myocardial cellsduring heat stress in vitro

摘要：

　　Neonatal rat primary myocardial cells were subjected to heat stressin vitro,as a model for investigating the distribution and expression of Hsp27 and αB-crystallin. After exposure to heat stress at 42 ℃ for different durations,the activities of enzymes expressed during cell damage increased in the supernatant of the heat-stressed myocardial cells from 10 min,and the pathological lsions were characterized by karyopyknosis and acute degeneration. Thus,cell damage was induced at the onset of heat stress. Immunofluorescence analysis showed stronger positive signals for both Hsp27 and αB-crystallin from 10 min to 240 min of exposure compared to the control cells. According to the Western blotting results,during the 480 min of heat stress,no significant variation was found in Hsp27 and αB-crystallin expression;however,significant differences were found in the induction of their corresponding mRNAs. The expression of these small heat shock proteins (sHsps) was probably delayed or overtaxed due to the rapid consumption of sHsps in myocardial cells at the onset of heat stress. Our findings indicate that Hsp27 and αB-crystallin do play a role in the response of cardiac cells to heat stress,but the details of their function remain to be investigated.

关键词： Hsp27 αB-crystallin heat stress myocardial cells

作者: Shu Tang Rehana Buriro Zhijun Liu Miao Zhang Islam Ali Abdelnasir Adam J(o)rg Hartung Endong Bao

作者单位: College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China College of Animal Science and Technology,Jinling Institute of Technology,Nanjing 210038,China Institute for Animal Hygiene,Animal Welfare and Farm Animal Behaviour,University of Veterinary Medic

会议类型: 国内会议

会议名称: 2013年中国畜牧兽医学会兽医病理学分会暨中国病理生理学会动物病理生理学专业委员会学术研讨会

会议地点: 南京

会议语种:英文

页码: 251-260

在线出版日期: 2013-08-01（万方平台首次上网日期，不代表论文的发表时间）