Expression and Distribution of Laminin Receptor Precursor/Laminin Receptor in Rabbit Tissues
The 37/67-kDa laminin receptor precursor (LRP)/laminin receptor (LR) is a cell surface receptor for cellular prion proteins and misfolded pathological prions. Previous research has shown that blocking or decreasing LRP/LP levels by anti-LRP/LR antibodies or small interfering RNAs (siRNAs) can prolong the incubation phase of experimental prion infection. This study aimed to investigate potential mechanisms contributing to prion resistance/susceptibility by using the rabbit,a species unsusceptible to prion infection,as a model. We investigated the expression level and distribution of LRP/LR in rabbit tissues by real-time polymerase chain reaction and by immunochemical analysis with a monoclonal anti-67 kDa LR antibody. Our results showed LRP/LR mRNA expression in all the tissues examined. Very low LRP/LR expression levels were observed in central nervous system (CNS) tissues, whereas high expression levels were observed in reproductive and digestive tissues,which differed from the expression patterns that have been reported for prionsusceptible animals. The immunochemical staining results were generally consistent with the mRNA findings,although no LR protein was detected in CNS tissues. Our findings provide a basis for further studies on prion resistance in rabbits and other animal species.
Laminin receptor precursor/laminin receptor Rabbit Expression Distribution Prion
Huinuan Wang Xiangmei Zhou Xiaomin Yin Yunsheng Wang Deming Zhao Lifeng Yang Mohammed Kouadir Rongrong Tan Wenyu Wu Huarong Zou Jin Wang Sher Hayat Khan Dongfeng Li
State Key Laboratories for Agrobiotechnology,Key Lab of Animal Epidemiology and Zoonosis,Ministry of State Key Laboratories for Agrobiotechnology,Key Lab of Animal Epidemiology and Zoonosis,Ministry of National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Br
国内会议
2013年中国畜牧兽医学会兽医病理学分会暨中国病理生理学会动物病理生理学专业委员会学术研讨会
南京
英文
285-295
2013-08-01（万方平台首次上网日期，不代表论文的发表时间）