Plasmodium yoelii:Correlation of TEP1 with mosquito melanization induced by nitroquine
The antimalarial drug nitroquine is not only an effective antimalarial drug,it is also able to induce the melanization of Piasmodium spedes.However,the molecular mechanisms of the recognition reaction induced by this drug remain unclear.Silencing of thioester-containing protein-1 (TEP1) significantly compromised the ability of Anopheles gambiae to melanize the Plasmodium,leading to investigation of the involvement of A stephensi TEP1 in melanization induced by nitroquine.This study shows that (1) binding of,AsTEP1 to oocysts,especially melanized oocysts,(2) after ingestion of anti-AsTEP1 antibody,the melanization rate in antibody-treated mosquitoes are significantly lower than in control mosquito (p < 0.05).The results suggest that nitroquine is able to induce Plasmodium recognition by TEP1,possibly triggering the resulting melanotic encapsulation.Further elucidation of the molecular mechanisms of mosquito immunity induced by antimalarial drugs will provide theoretical evidence for the use of anti malarial drugs,and a meaningful pathway for the design of novel antimalarial drugs.
Nitroquine Melanization Anopheles stephensi Plasmodium yoelii TEP
Jian Zhang Fu Sheng Huang Wen Yue Xu Ying Wang Tao Li Zhou Jian Hua Duan
Department of the Pathogenic Biology, The Third Military Medical University, Chongqing 400038, PR China
国内会议
重庆
英文
178-183
2012-10-20（万方平台首次上网日期，不代表论文的发表时间）