A novel approach to study the metabolism of γ-tocopherol in the liver post-mitochondrial supernatant of male SD-rat
A novel approach was employed to study the metabolism of γ-tocopherol in the liver post-mitochondrial supernatant (S9) of male Sprague-dawley rat (Rattus norvegiens, SD-rat).An optimal reactive system of γ-tocopherol metabolism was composed of 5 mg/ml of S9, 8 μg/ml of γ-tocopherol, 1 mmol/ml of Nicotine amide adenine dinucleotide phosphate (NADPH), and 45 min of reactive time at 37℃.The metabolites of γ-tocopherol were analyzed using gradient elution and reverse-phase high-performance liquid chromatography (RP-HPLC).The structure of γ-tocopherol metabolites was determined using gas chromatography-mass spectrometry (GC-MS) and a main metabolite was identified as ω-hydroxylate, which is a side chain of γ-tocopherol and a possible form of the vitamin that would be most utilized by SD-rat.
Metabolism post-mitochondrial supernatant (S9) SD-rat γ-tocopherol
Youqing Xu Zhaokun Ding
Faculty of Animal Sciences and Technology, Guangxi University, 530004 Nanning,China
国内会议
拉萨
英文
74-85
2012-09-14（万方平台首次上网日期，不代表论文的发表时间）