Lowered Nudix type 5 (NUDTS) expression leads to cell cycle retardation in HeLa cells
The molecule 8-Oxo-7, 8-dihydroguanine (8-oxoGua), an oxidized form of guanine, can pair with adenine or cytosine during nucleic acid synthesis.RNA containing 8-oxoGua causes translational errors leading to abnormal proteins.Human NUDT5, a MutT-related protein, catalyzes the hydrolysis of 8-oxoGDP to 8-oxoGMP, thereby preventing misincorporation of 8-oxoGua into RNA.To investigate the biological roles of NUDT5 in mammalian cells, we established cell lines with decreased levels of NUDT5 expression.In NUDT5 knockdown cells, the RNA oxidation level was not significantly higher than that of normal cells, howerver, the cell cycle G1 phase was significantly delayed, and cell numbers in both the S and G2/M phases were reduced, indicating that cell proliferation was hampered by NUDT5 knockdown.Key proteins for preventing the G1-S transition, such as p53, p16 and Rb were increased, while the phosphorylation of Rb was decreased.These results suggested that the NUDT5 protein may play significant roles in regulating the G1-S transition in mammalian cells.
NUDT5 RNAi RNA oxidation cell cycle retardation
Li-qun Zhang Da-peng Dai Yan-yang Zhao Wei Gan Yasumitsu Takagi Hiroshi Hayakawa Mutsuo Sekiguchi Jian-Ping Cai
Graduate School,Peking Union Medical College & Chinese Academy of Medical Sciences,Beijing 100730,P. The Key Laboratory of Geriatrics,Beijing Hospital & Beijing Institute of Geriatrics,Ministry of Heal Advanced Research Center,Fukuoka Dental College,Fukuoka 814-0193,Japan
国内会议
第八届全国“体外诊断学新技术、新方法、新概念、新进展”学术研讨会暨第二届“阳普医疗杯”临床检验诊断学硕博论坛
北京
英文
170-196
2011-09-01（万方平台首次上网日期，不代表论文的发表时间）