Determination of amino acids in the traditional Chinese medicine formula Sheng-Mai-San by high performance capillary electrophoresis

摘要：

　　A method was developed for the quantitative analysis of amino acids in Sheng-Mai-San (SMS), using micellar electrokinetic chromatography (MEKC) in uncoated fused silica capillaries and UV detection.The influence of different parameters, such as the organic modifier and applied voltage on the MEKC separation of amino acids was investigated.A good compromise between resolution and migration time was obtained by use ofa 70mM SDS on borate buffer (pH 9.5, I=40mM) with 15% acetonitrile.That method was applied to determine amino acid in SMS and SMS market formulas samples.

关键词： Sheng-Mai-San Free amino acids analysis High performance capillary electrophoresis

作者: Na Xu Boyang Yu Yongqing Yan Danni Zhu

作者单位: Department of Complex Prescription of TCM,,China Pharmaceutical University,NO.639,Longmian Road,Nanj Department of Complex Prescription of TCM,,China Pharmaceutical University,NO.639,Longmian Road,Nanj

会议类型: 国内会议

会议名称: 第四届华东地区色谱、质谱学术报告会

会议地点: 无锡

会议语种:英文

页码: 236-241

在线出版日期: 2010-11-04（万方平台首次上网日期，不代表论文的发表时间）