Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm

摘要：

　　Tumor heterogeneity presents a challenge for infer ring clonal evolution and driver gene identification.Here, we describe a method for analyzing the cancer genome at a single-cell nucleotide level.To perform our analyses, we first devised and validated a high-throughput whole-genome single-cell se quencing method using two lymphoblastoid cell line single cells.We then carried out whole-exome single-cell sequencing of 90 cells from a JAK2-negative myeloproliferative neoplasm patient.The sequencing data from 58 cells passed our quality control criteria, and these data indicated that this neoplasm represented a monoclonal evolution.

作者: Yong Hou Di Shao Hanjie Wu Xiaofei Ye Chen Ye Renhua Wu Min Jian Yan Chen Wei Xie Ruren Zhang Lei Chen Luting Song Xin Liu Xiaotian Yao Hancheng Zheng Chang Yu Qibin Li Zhuolin Gong Mao Mao Xu Yang Lin Yang Jingxiang Li Ping Zhu Wen Wang Zuhong Lu Ning Gu Goodman Laurie Lars Bolund Karsten Kristiansen Jian Wang Huanming Yang Yingrui Li Xiuqing Zhang Bo Zhang Jun Wang Ye Tao Xun Xu Fuqiang Li Kui Wu Jie Liang

作者单位: BGI-Shenzhen, Shenzhen, 518083, China;State Key Laboratory of Bioelectronics;School of Biological Sc BGI-Shenzhen, Shenzhen, 518083, China Department of Hematology, Peking University First Hospital, Beijing 100034, China BGI-Shenzhen, Shenzhen, 518083, China;School of Biological Science and Medical Engineering Southeast BGI-Shenzhen, Shenzhen, 518083, China;College of Life Sciences, Wuhan University, Wuhan 430072, Chin BGI-Shenzhen, Shenzhen, 518083, China;College of Life Sciences, Wuhan University, Wuhan 430072, Chin Pfizer Inc., San Diego, CA92121, USA Department of Hematology, Peking University First Hospital, Beijing 100034, China;These authors cont CAS-Max Planck Junior Research Group, State Key Laboratory of Genetic Resources and Evolution, Kunmi State Key Laboratory of Bioelectronics;School of Biological Science and Medical Engineering Southeas BGI-Shenzhen, Shenzhen, 518083, China;Departrnent of Biology BGI-Shenzhen, Shenzhen, 518083, China;These authors contributed equally to this work BGI-Shenzhen, Shenzhen, 518083, China;Departrnent of Biology;The Novo Nordisk Foundation Center for

会议类型: 国内会议

会议名称: 第八届全国难治性白血病学术研讨会、第四届全国难治性淋巴瘤学术研讨会、第四届全国多发性骨髓瘤学术研讨会、第五届钟山国际MDS学术研讨会、南京2012血液学年会

会议地点: 南京

会议语种:英文

页码: 166-178

在线出版日期: 2012-05-01（万方平台首次上网日期，不代表论文的发表时间）