Reversal Effect of Gambogic Acid on Multidrug Resistance in K562/A02 Cell Line
This study was purposed to investigate the multidrug resistance reversal effect of Gambogic Acid in K562/AO2 cells and its mechanism.The IC50 of ADM was evaluated by MTT.Cell apoptosis was detected by flow cytometry.Morphological changes of K562/A02 cells stained by DAPI under fluorescence microscope.The expressions of Survivin and P-gp were determined by Western blot.The results showed that the ICS0 of ADM in K562 and K562/AO2 cells were 1.42 ± 0.07μg/ml and 28.42±1.40μg/ml respectively.If the concentration of GA was less than 0.0625μmol/L, the inhibitory effect on proliferation of K562 and K562/A02 cells was not observed.0.06251μmol/L GA could enhance the sensitivity of K562/A02 cells to ADM (p < 0.05) and the reversal multiples was 1.53.The apoptotic rate was raised after treating with ADM combined with 0.0625μmol /L GA for 48 hours (p<0.05).
TIAN Liang LIU Ran CHEN Bao-An CHENG Jian DING Jia-Hua XIA Guo-Hua ZHANG Hai-Jun Wang Lei Ren Yan-Yan CAI Xiao-Hui
Department of Hematology and Oncology(the Key Subject of Jiangsu Province, Zhongda Hospital,Clinical Department of Hematology and Oncology(the Key Subject of Jiangsu Province, Zhongda Hospital,Clinical
国内会议
第八届全国难治性白血病学术研讨会、第四届全国难治性淋巴瘤学术研讨会、第四届全国多发性骨髓瘤学术研讨会、第五届钟山国际MDS学术研讨会、南京2012血液学年会
南京
英文
189-189
2012-05-01（万方平台首次上网日期，不代表论文的发表时间）