ABCG2 monoclonal antibody in combination with NPs-PTX in vitro inhibited multiple myeloma cancer stem cells
Objective To detect the inhibitive effect on multiple myeloma (MM) cancer stem cells(CSCs) by ABCG2 monoclonal antibody (McAb) in combination with Fe3O4 nanoparticle-paclitaxel(NPs-PTX) in vitro.Mothods MM CSCs were sorted from human MM cell lines NCI-H929 and RPMI8226 through the immune magnetic beads sorting method.Growth inhibitive effect was detected by CCK-8 assay.Effects of cell cycles and apoptosis were de tected by flow cytometry.Results The proliferation of NCI-H929 and RPMI8226 cells were suppressed after treat ment with ABCG2 McAb in combination with NPs-PTX for 24 hours.There is statistical significant difference between the combined utilization of ABCG2 McAb and NPs-PTX group in contrast to the utilization of NPs-PTX group alone.
YANG Cui-ping CHEN Bao-an XIONG fei DOU Jun LIU Yun-jin CHEN Jun-song GU Ning
Department of Pathogenic Biology and Immunology, Medical School, Southeast University Department of Hematology and Oncology(the Key Subject of Jiangsu Province) School of Biological Science & Medical Engineering, Southeast University,Nanjing
国内会议
第八届全国难治性白血病学术研讨会、第四届全国难治性淋巴瘤学术研讨会、第四届全国多发性骨髓瘤学术研讨会、第五届钟山国际MDS学术研讨会、南京2012血液学年会
南京
英文
220-220
2012-05-01（万方平台首次上网日期，不代表论文的发表时间）