Gene expression profiling in respond to TBT exposure in small abalone Haliotis diversicolor
In this study, we investigated the gene expression profiling of small abalone, Haliotis diversicolor by tributyltin (TBT) exposure using a cDNA microarray containing 2473 unique transcripts.Totally, 107 up-regulated genes and 41 down-regulated genes were found.For further investigation of candidate genes from microarray data and EST analysis, quantitative real-time PCR was performed at 6 h, 24 h, 48 h, 96 h and 192 h TBT exposure.26 genes were found to be significantly differentially expressed in different time course, 3 of them were unknown.Some gene homologues like cellulose, endo-beta-1,4-glucanase, ferritin subunit 1 and thiolester containing protein Ⅱ CG7052-PB might be the good biomarker candidate for TBT monitor.The identification of stress response genes and their expression profiles will permit detailed investigation of the defense responses of small abalone genes.
gene expression TBT microarray EST qRT-PCR Haliotis disversicolor
Xiwei Jia Zhihua Zou Guodong Wang Shuhong Wang Yilei Wang Ziping Zhang
The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen, Fujian, China, 361021
国内会议
厦门
英文
20-20
2011-11-15（万方平台首次上网日期，不代表论文的发表时间）