Using the Doubly Charged Selected Ion Monitoring(DC-SIM)Mode for the Identification of Gelatins Species
Gelatin is a mixture of high molecular weight polypeptides from the hydrolysis of collagen.Doubly Charged Selected Ion Monitoring (DC-SIM) is the most suitable scanning mode to detect known peptides in complex samples when an ion–trap mass spectrometer is the instrument used for the analysis.In this mode,the MS detector is programmed to perform continuous MS/MS on one or more selected precursors,either during a selected time interval,or along the whole chromatographic run.MS/MS spectra are recorded,so virtual multiple reaction monitoring chromatogram traces for the different fragment ions can be plotted.In this work,a PCA method was applied to the detection of previously characterized species-specific peptides from different gelatins.The proposed methodology makes use of trypsin digestion for sample preparation,peptide separation and identification by rapid resolution liquid chromatography coupled to an ion-trap working in the auto MS2 mode.This methodology was applied to the differential classification of five commercial,homological,species of gelatins proving to be an excellent tool for gelatins product authentication,which may be used by manufacturers to provide a fast and effective identification of the gelatins,guaranteeing the quality and safety of foodstuffs to consumers.
Selected ion tandem mass spectrometry gelatin polypeptides and identification
CHENG Xianlong LI Wenjie ZHANG Xiaolong WEI Feng XIAO Xinyue ZHANG Li LIN Ruichao MA Shuangcheng
National Institutes for Food and Drug Control,Beijing 100050,China;Beijing University of Chinese Med National Institutes for Food and Drug Control,Beijing 100050,China Lianyungang Institute for Drug Control,Lianyungang 222006,China Beijing University of Chinese Medicine,Beijing 100102,China
国内会议
贵阳
英文
1-8
2013-05-01（万方平台首次上网日期，不代表论文的发表时间）