Therapeutic Time Window and Underlying Therapeutic Mechanism of Breviscapinun Injection against Cerebral Ischemia/Reperfusion Injury in Rats
Our study was designed to investigate the therapeutic time window and underlying therapeutic mechanism of breviscapinun injection against cerebral ischemic / reperfusion injury.In the present study,Sprague-Dawley rats were subjected to middle cerebral artery occlusion for 2 h.Rats were intravenous injected with 40 mg/kg breviscapinun injection at different time-points and the same doses were administered every 24 h for 3 consecutive days.After 72 h of reperfusion,neurologic scores,infarct volume and the levels of neuron-specific enolase were measured in a masked fashion.To explore the role of HIF-1α in the neuroprotective effects of breviscapinun injection,immunohistochemistry and western blot analysis in a separate study were performed after 3 and 24 h of reperfusion.Treatment with breviscapinun significantly ameliorated neurologic deficit,reduced infarct volume and suppressed the NSE levels in a time-dependent manner.Moreover,treatment with breviscapinun increased the expression of HIF-1α protein at 3 and 24 h after reperfusion.These results suggest that the therapeutic time window of breviscapinun injection for cerebral ischemia / reperfusion injury seem to be within 5 h after reperfusion,and by increasing the expression of HIF-1α may be involved in the therapeutic mechanism of breviscapinun.
breviscapinun ischemia/reperfusion therapeutic time window hypoxia-inducible factor-1
GUO Chao ZHU Yanrong QUAN Wei YIN Ying Xi Miaomiao ZHOU Dan WEN Aidong
Fourth Military Medical University,Xi’an 710032,China
国内会议
贵阳
英文
1-9
2013-05-01（万方平台首次上网日期，不代表论文的发表时间）