Preventative effects of Ginkgo biloba extract (EGb761) on high glucose-cultured opacity of rat lens
Aim: To investigate the protective effects of EGb761 on high glucose-induced opacity of rat lens.Methods: After rat lenses were excised from the eyes, lenses were divided into 6 groups: normal group, high glucose group, low-, moderate-, and high-dose of EGb761 groups, and bendazac lysine group.The activities of antioxidases, aldose reductase (AR), advanced glycosylation end products (AGEs), transforming growth factor-β2 (TGF-β2), Smad2/3, E-cadherin, and α-SMA were assessed.Results: Compared with in high glucose group, EGb761 relieved oxidative stress, decreased AR activation and AGEs level, and suppressed the TGF-β2/Smad pathway activation, further increase the expression of E-cadherin and decrease α-SMA, and therefore prevents the pathological changes of high glucose-induced lens epithelial cells and ameliorated lens opacity.Conclusion: EGb761 has protective effects on several pharmacological targets in the progress of diabetic cataract and is a potential drug for the prevention of diabetic cataract.
Ginkgo biloba extract lens opacity high glucose oxidative stress TGF-β2
Qian Lu Xiaoxing Yin Tingting Yang Mingzhu Zhang Lei Du Ling Liu Nan Zhang Fan Zhang Hao Guo Gang Hu
Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology.Nanjing Medical University, Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology.Nanjing Medical University, Laboratory of New Drugs and Clinical Application, Xuzhou Medical College, Xuzhou, 221004,China
国内会议
江苏省药理学会青年工作委员会成立大会暨药理学科青年科技创新学术研讨会
苏州
英文
95-107
2013-05-24（万方平台首次上网日期，不代表论文的发表时间）