MiR-17-3p inhibits angiogenesis by down-regulating Flk-1 in the cell growth signal pathway
MicroRNAs are endogenously expressed small noncoding RNAs that regulate gene expression on the posttranscriptional level.Previous works indicated that miR-17-92 cluster could regulate endothelial cells (Ecs) functions involved in angiogenesis.We have identified microRNA-17-3p (miR-17-3p), a component ofmiR-17-92 cluster, could control the angiogenic activity of human umbilical vein endothelial cells (HUVECs) in a cellautonomous manner in vitro.A 21-bp fragment from Flk-1 3”-untranslated region (3”-UTR) containing miR-17-3p targeting sites was required for the rapid down-regulation of Flk-1 expression by in silico and experimental analysis.Subsequently, the downstream of cell growth pathway was inhibited by forced up-regulation of miR17-3p.Based on these data, we conclude that miR-17-3p is a negative regulator of angiogenic phenotype of Ecs through its ability to modulate the expression of Flk-1, which is implicated the pleiotropie effects of miR-17-92 in angiogenesis.
Runting Yin Wei Zhang
Department of Pharmacology, Nantong University Medical College, Nantong, P.R.China
国内会议
江苏省药理学会青年工作委员会成立大会暨药理学科青年科技创新学术研讨会
苏州
英文
175-175
2013-05-24（万方平台首次上网日期，不代表论文的发表时间）