Retrograde Coronary Venous Delivery of Fibroblast Growth Factor-2 Enhances in Vivo Homing and Differentiation of Mesenchymal Stem Cells
Objectives In vitro studies showed fibroblast growth factor-2 (FGF-2) controls migration of mesenchymal stem cells (MSCs)In the present study,we established concentration gradient of FGF-2 between coronary venous blood and target myocardium by coronary venous retroperfusion and assessed the effects of FGF-2 gradient on in vivo homing and differentiation of MSCs.Methods Acute myocardial infarction (AMI) was induced by ligation of left anterior descending (LAD) coronary artery.First,twelve animals were randomized into 4 groups based on the time of balloon dilation (retroperfusion time),being 0 min (n=3),5 min (n=3),10min (n=3),15min (n=3).One week after AMI,FGF-2 was retrogradely perfused into anterior interventricular vein in parallel to LAD,and FGF-2 concentrations of serum and myocardial tissue were measured by ELISA.The time of FGF-2 gradients after coronary venous retroperfusion was evaluated.Second,ten animals were randomized to MSCs group (n=5) and FGF-2+MSCs group (n=5) after AMI.MSCs were cultured and transfected by pLEGFP-N1 vector before transplantation.One week after retroperfusion,EGFP-positive endothelial cells and cardiomyocytes in infarcted myocardium were compared between the two groups by immunofluorescence method.
Cardiac regeneration Growth factors Stem cells
Shaoping Nie Xiao Wang Shibin Qiao Lei Zhen Jianzeng Dong Xiaohui Liu Changsheng Ma
Beijing Anzhen Hospital, Capital Medical University, Beijing
国内会议
北京
英文
63-63
2013-04-19（万方平台首次上网日期，不代表论文的发表时间）