The therapeutic time window of 25% xenon postconditioning to protect against spinal cord ischemia-reperfusion injury in rats
Aims Spinal cord isehemia reperfusion insults remain a major cause of acute and chronic paraplegia in patients undergoing aortic surgery.We and others have demonstrated that preconditioning or posteonditioning with xenon at 70% (v/v) or 50% (v/v),respectively,has a neuroprotective effect in both rat and rabbit models of ischemia reperfusion injury (IRI).Therefore,the present study was designed to assess the neuroprotective effect and therapeutic time window of 25% xenon postconditioning on spinal cord ischemia-reperfusion injury (IRI) in the rat.Methods Four groups of rats (IRI control and three xenon postconditioning groups,n=10 per group) underwent spinal cord IRI with aortic occlusion.Controls inhaled 25% nitrogen for lh,from reperfusion.The three postconditioning groups inhaled 25% xenon for 1 h,either starting at reperfusion or 1 or 2 h after reperfusion.Shams (n=10) underwent the same surgical procedure without aortic occlusion and inhaled the same gas as control rats.Neurologic function was assessed using the Basso,Beattie,and Bresnahan score,4,24,and 48 h after reperfusion.Expression of spinal cord pro (Bax) and antiapoptotic (Bcl2) proteins was assessed using immunohistochemistry,and apoptosis was observed by TUNEL and Nissl stainine.
xenon postconditioning the therapeutic window
Yang YW Lu JK Qing EM Dong XH Wang CB Zhang J Zhao LY Gao ZF Cheng
Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel DiseascsBlood Vessel Diseases
国内会议
北京
英文
108-108
2013-04-19（万方平台首次上网日期，不代表论文的发表时间）