Celastrus orbiculatus extract inhibits tumor angiogenesis by targeting VEGF signaling pathways and shows potent antitumor activity in hepatocarcinomas in vitro and in vivo
ABSTRACT Objective Celastrus orbiculatus Thunb has been used for thousands of years in China as a remedy against cancer and inflammatory diseases.This study aims to investigate whether C.orb iculatus extract (COE) could inhibit angiogenesis,which is the pivotal step in tumor growth,invasiveness,and metastasis.Methods In this study,the extract from the stem of Celastrus orbiculatus was used.Mouse hepatic carcinoma cells (Hepal-6) were treated with COE in different nontoxic concentrations (10,20,40,80,and 160 μg/mL).The mRNA and protein expression levels of vascular endothelial growth factor (VEGF) were detected by RT-PCR and Western Blot,respectively; the active fractions were further tested on C57BL/6 mice and human umbilical vein endothelial cells (HUVEC) for any anti-angiogenic effects.Results COE significantly inhibited proliferation and induced apoptosis in Hepal-6 cells and inhibited VEGF expression at both the mRNA and protein levels.Furthermore,this agent inhibited the formation of the capillary-like structure in primary cultured HUVEC in a dose-dependent manner.In vivo,COE significantly reduced the volume and weight of solid tumors with low adverse effects,and it decreased tumor angiogenesis.Conclusions In summary,COE could be used to treat hepatic carcinoma.The mechanisms of the antitumor activity of COE may possibly be due to its effects against tumor angiogenesis by targeting the VEGF protein.
antitumor Celastrus orbiculatus Thunb. angiogenesis VEGF
Yayun Qian Hua Zhang Ying Hou Lin Yuan Guoqing Li Shiyu Guo Hisamits Tadashi Yanqing Liu
Institute of Traditional Chinese Medicine & Western Medicine, School of Medicine, Yangzhou Universit Institute of Traditional Chinese Medicine & Western Medicine, School of Medicine, Yangzhou Universit Department of Physiology, School of Medicine, Showa University, 1-5-8 Hatanodai, Tokyo 142-8555, Jap
国内会议
南京
英文
17-27
2012-07-01（万方平台首次上网日期，不代表论文的发表时间）