Electrical Field Stimulation Induces Cardiac Fibroblast Proliferation through Calcineurin-NFAT pathway
Aim:To observe the effects and potential mechanisms of electrical field stimulation (EFS) on cardiac fibroblasts (CFs) proliferation Methods:Cardiac fibroblasts were isolated from the neonatal SD rats and cultured in Dulbecco”s modified Eagle”s medium (DMEM),cells of passage 3 were used for experiments.EFS was used as an irritant agent.CFs proliferation was assessed by a cell survival assay (MTT) and cell counts.Myocardial fibrosis was assessed by checking the expression of collagen Ⅰ and Ⅲ by western blotting.CaN activity was determined by activity assay kit.Nuclear factor of activated T cell (NFAT) isoforms expression was determined by RT-PCR.Green fluorescent protein (GFP)-tagged NFAT was used to detect NFAT nuclear translocation.
Qingqing Chen Wei Zhang Weizhong Zhu
Department of Pharmacology, School of Medicine, Nantong University, Nantong, China
国内会议
南京
英文
41-42
2012-07-01（万方平台首次上网日期，不代表论文的发表时间）